hepaticus colonization and its association with pathological features by establishing BALB/cCr Mice Model with H.hepaticus infection. Methods: SPF male BALB/c Cr mice were inoculated H.hepaticus standard strain ATCC51450. The

control group was fed with PBS. Mice were executed at 1st, 3rd, 6th, 9th and 12th month after the last inoculation. Serum were taken for H.hepaticus-IgG and mice esophagus, stomach, jejunum, ileum, cecum, colon, liver and pancreas tissue were taken for histopathology examiantion, isolation culture and H.hepaticus specific 16S rRNA gene amplification. Results: The seroprevalance of H.hepaticus-IgG antibody in BALB/c Cr mice infected with H.hepaticus were all 100% from buy Acalabrutinib 1st months to 12th month. Antibody level reached peak value at 6th month, then gradually decline. The colonization rate of H.hepaticus in cecum at 1st month were 80%, then continued colonization in cecum, colonization rate in cecum from 3 to 12 months were 100%. H.hepaticus colonization in liver was detected at 3th month, and colonization rate varies between 20∼40%; Colonization of H.hepaticus in esophagus, stomach

and pancreas tissue were not detected; The colonization of H.hepaticus in digestive tract tissue in control Pritelivir supplier group were not detected. Liver histopathologic scores were gradually increased as infection time extended within 6 months. There were no significant differences in liver histopathologic scores from 6th month to 12th month. The histopathologic scores in cecum and colon at 3th month were higher than those at 1st month. Three were no significant differences in cecum and colon histopathologic scores from 3th month to 12th month. Conclusion: The colonization site of mice infected with H.hepaticus is lower digestive tract and liver, cecum is the site of initial colonization; H.hepaticus could

induced not only digestive tract diseases, but also liver injury. Histological scores were gradually increased as infection time extended. Key Word(s): 1. BALB/c mice; 2. Histopathology; 3. Colonization; 4. H.hepaticus-IgG; Presenting Author: ANJIANG WANG Additional Authors: JIAN WANG, BIMIN find more LI, ZHIJIAN LIU, LU CHEN, HE WANG, FENG SHI, XUAN ZHU Corresponding Author: XUAN ZHU Affiliations: The first affiliated hospital of Nanchang University Objective: There is no study verifying the value of MELD-Na (model for end-stage liver disease and sodium) in predicting rebleeding and associated mortality in cirrhotic patients after cessation of initial esophageal variceal hemorrhage. This study was aimed to determine whether MELD-Na would be more accurate in predicting rebleeding and associated mortality than other models such as MELD or Child-Turcotte-Pugh (CTP).