2003;24:1681–91.PubMedCrossRef 29. Corrales-Garcia LL, Possani LD, Corzo G. Expression systems of human β defensins: vectors, purification and
biological activities. Amino Acids. 2011;40:5–13.PubMedCrossRef 30. Taggart CC, Greene CM, Smith SG, et al. Inactivation of human beta-defensins 2 and 3 by elastolytic cathepsins. J Immunol. 2003;171:931–7.PubMed 31. Smith EE, Buckley DG, Wu Z, et al. Genetic adaptation by Pseudomonas aeruginosa to the airways of cystic fibrosis patients. Proc Natl Acad Sci USA. 2006;103:8487–92.PubMedCentralPubMedCrossRef 32. Jelsbak L, Johansen HK, Frost AL, et al. Molecular epidemiology and dynamics of Pseudomonas Fer-1 aeruginosa populations in the lungs of cystic fibrosis patients. Infect Immun. 2007;75:2214–24.PubMedCentralPubMedCrossRef 33. Cobb LM, Mychaleckyj JC, Wozniak DJ, Lopez-Boado YS. Pseudomonas aeruginosa flagellin and alginate elicit very different gene expression Selleck TPCA-1 patterns in airway epithelial cells:
implications for cystic fibrosis disease. J Immunol. 2004;173:5659–70.PubMed 34. Soutourina OA, Bertin PN. Regulation cascade of flagellar expression in Gram-negative bacteria. FEMS Microbiol Rev. 2006;274:505–23. 35. Hayashi F, Smith KD, Ozinsky A, et al. The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5. Nature. 2001;410:1099–103.PubMedCrossRef 36. Chow JC, Young DW, Golenbock DT, Christ WJ, Gusovsky F. Toll-like receptor-4 mediates lipopolysaccharide-induced signal transduction. J Biol Chem. 1999;288:10689–92.CrossRef 37. Wu Q, Lu Z, Verghese MW, Randell SH. Airway epithelial check details cell tolerance to Pseudomonas aeruginosa. Respir Res. 2005;6:26.PubMedCentralPubMedCrossRef 38. Wehkamp J, Harder J, Wehkamp K, et al. NF-kappaB- and AP-1-mediated induction of human beta
defensin-2 in intestinal epithelial cells by Escherichia coli Selleck Fluorouracil Nissle 1917: a novel effect of a probiotic bacterium. Infect Immun. 2004;72:5750–8.PubMedCentralPubMedCrossRef 39. Chen CI, Schaller-Bals S, Paul KP, Wahn U, Bals R. Beta-defensins and LL-37 in bronchoalveolar lavage fluid of patients with cystic fibrosis. J Cyst Fibros. 2004;3:45–50.PubMedCrossRef 40. MacRedmond R, Greene C, Taggart CC, McElvaney N, O’Neill S. Respiratory epithelial cells require Toll-like receptor 4 for induction of human beta-defensin 2 by lipopolysaccharide. Respir Res. 2005;6:1–11.CrossRef 41. Greene CM, Carroll TP, Smith SG, et al. TLR-induced inflammation in cystic fibrosis and non-cystic fibrosis airway epithelial cells. J Immunol. 2005;174:1638–46.PubMed 42. Baggiolini M, Dewald B. The neutrophil. Int Arch Allergy Immunol. 1985;76:13–20.CrossRef 43. Doring G. The role of neutrophil elastase in chronic inflammation. Am JRespir Crit Care Med. 1994;150:S114–7.CrossRef 44. Dunlevy FK, Martin SL, de Courcey F, Elborn JS, Ennis M. Anti-inflammatory effects of DX-890, a human neutrophil elastase inhibitor. J Cyst Fibros. 2012;11:300–4.PubMedCrossRef 45. Jensen PO, Bjarnsholt T, Phipps R, et al.