26 0.9411 -0.3480 UD UD UD UD UD UD UD UD UD UD P17 CLIBASIA_03110 20.11 0.9994 -0.2786 UD UD UD UD UD UD UD UD UD UD P18 CLIBASIA_03675 20.02 0.9967 -0.2780 UD UD UD UD UD UD UD UD UD UD P19 CLIBASIA_03725 19.91 NT NT 35.29 UD UD UD UD UD UD UD UD UD P20 CLIBASIA_03955 21.08 NT NT UD UD UD UD 37.41 UD UD UD UD UD P21 CLIBASIA_04030 20.30 NT NT UD UD UD UD 32.93 UD UD UD UD UD P22 CLIBASIA_04150 24.00 NT NT UD UD UD UD UD UD UD UD UD UD P23 CLIBASIA_04310 20.76 0.991 -0.2976 UD UD UD UD UD UD UD UD UD UD P24 CLIBASIA_04330 20.85 0.9986 -0.2635 UD UD UD UD UD UD UD UD UD UD P25 CLIBASIA_04405 21.60 0.9987 -0.3051 UD UD UD UD UD UD UD UD UD UD P26 CLIBASIA_04425 20.41 0.9994 -0.3032 UD CB-839 clinical trial UD UD UD

UD UD UD UD UD UD P27 CLIBASIA_02645 21.77 NT NT 38.61 UD UD UD UD UD UD UD UD UD P28 CLIBASIA_04515 22.00 NT NT 38.63 UD UD UD UD UD UD UD UD UD P29 CLIBASIA_04530 19.00 0.9919 -0.2852 UD UD UD UD UD UD UD UD UD UD P30 CLIBASIA_04550 22.48 0.9938 -0.2708 UD UD UD UD UD UD UD UD UD UD P31 CLIBASIA_05230 21.68 0.9941 -0.2771 UD UD UD AR-13324 UD

UD UD UD UD UD UD P32 CLIBASIA_05480 21.48 0.988 -0.2776 UD UD UD UD UD UD UD UD UD UD P33 CLIBASIA_04475 20.84 0.9913 -0.2644 UD UD UD UD NT UD UD UD UD UD P34 CLIBASIA_05505 22.70 0.9893 -0.2791 UD UD UD UD NT UD UD UD UD UD CQULA04F/R β-operon 22.11 NT NT UD UD UD UD NT NT NT NT NT NT LJ900f/r Prophage 22.25 NT NT UD UD UD UD NT NT NT NT NT NT HLBas/r 16Sas 24.33 0.9998 -0.3057 NT NT UD UD NT NT NT NT NT NT HLBam/r 16Sam NT NT NT NT 24.68 UD UD NT NT NT NT NT NT HLBaf/r 16Saf NT NT NT 21.28 NT UD UD NT NT NT NT NT NT COXf/r Cox 14.80 NT NT 15.21 18.54 16.15 UD NT NT NT NT NT NT †Las-infected psyllids total DNA was serially diluted spanning up to five logs and used as a template in the qRT-PCR assay. R2 and ifenprodil slope were further calculated from a plot of CT values versus log dilution factor. Most of our novel custom designed primer pairs targeting the unique gene sequences were indeed found to be highly specific to Las, as assessed by qRT-PCR assays (Table 1). Among the 34 primer pairs, 29 produced amplicons only when Las-infected click here citrus plant DNA was used as a template, with an average CT value ranged from 19.48 to 27.47. Two primer pairs, P13 and P15, didn’t produce any amplicons under the standard conditions tested.