35), HLA-B*08 was more prevalent in patients with than without IBD (35% vs 6%, P=0.03), while IBD did not define genetically different subgroups in patients with low IgG4.Conclusions: We report for the first time on genetic associations for elevated IgG4 levels in PSC. Our findings suggest that the phenotypic heterogeneity represented by the IgG4 response contribute to the complex HLA associations observed in PSC. The possibility that elevated IgG4 concentration designate a genetic as well as clinical subgroup should be taken into account in further studies, ultimately aiming to identify patients suitable for immunosuppressive therapy or other treatment
options. Disclosures: The following people have nothing to disclose: Natalie L. Berntsen, Olav Klingenberg, Kirsten CT99021 cell line M. Boberg, Tom H. Karlsen, Johannes learn more R. Hov “
“Aims: This study is to elucidate whether cyclic adenosine monophosphate (cAMP)-mediated signal is involved in lower regenerative potential of cirrhotic liver. Methods: Hepatic cAMP concentration, activities of protein kinase A (PKA), c-AMP responsive element binding protein (CREB) and Ca2+-independent phospholipase A2 (iPLA2) and regeneration rate were compared between rats with thioacetamide-induced
cirrhotic and normal livers after two-third hepatectomy. Results: The liver regeneration estimated by the rates of [3H]-thymidine incorporation and staining of proliferating cell nuclear antigen was significantly lower in the cirrhotic group. CREB, PKA and iPLA2 activities, assessed by western blots and electromobility shift assay, were significantly impaired after hepatectomy in the cirrhosis group. PKA and iPLA2 silencing by siRNA transfection significantly inhibited CREB activity and cell growth in transformed hepatocytes in vitro. Conclusions: CREB dysfunction, mediated by PKA and iPLA2 suppression, may be involved in the deteriorated liver regeneration in the cirrhotic rats. “
“Department of Immunology,
University of Washington, Seattle, WA Department of Biology, University of Washington, Seattle, WA Silymarin, an extract from milk thistle (Silybum marianum), and its purified flavonolignans have been recently shown to inhibit hepatitis C virus (HCV) MCE公司 infection, both in vitro and in vivo. In the current study, we further characterized silymarin’s antiviral actions. Silymarin had antiviral effects against hepatitis C virus cell culture (HCVcc) infection that included inhibition of virus entry, RNA and protein expression, and infectious virus production. Silymarin did not block HCVcc binding to cells but inhibited the entry of several viral pseudoparticles (pp), and fusion of HCVpp with liposomes. Silymarin but not silibinin inhibited genotype 2a NS5B RNA-dependent RNA polymerase (RdRp) activity at concentrations 5 to 10 times higher than required for anti-HCVcc effects. Furthermore, silymarin had inefficient activity on the genotype 1b BK and four 1b RDRPs derived from HCV-infected patients.