Certain pumps may shed extrinsic particles that may lead to heterogeneous nucleation-induced aggregation. In this work, microflow imaging, size-exclusion chromatography (SEC), and turbidimetry were utilized to quantify subvisible IPI-145 price particles, aggregation, and opalescence, respectively. The filling process was performed using

several commonly used filling systems, including rotary piston pump, rolling diaphragm pump, peristaltic pump, and time-pressure filler. The rolling diaphragm pump, peristaltic pump, and time-pressure filler generated notably less protein subvisible particles than the rotary piston pump, although no change in aggregate content by SEC was observed by any pump. An extreme increase in subvisible particles was also reflected in an increase in turbidity. (C) 2011 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 100: 4198-4204, 2011″
“We describe a unique extracellular matrix (ECM) niche in the spleen, the marginal

zone (MZ), characterized by the basement membrane glycoproteins, laminin alpha 5 and agrin, that promotes formation of a specialized population of MZ B lymphocytes that respond rapidly to blood-borne antigens. Mice with reduced laminin alpha 5 expression show reduced MZ B cells and increased numbers Batimastat of newly formed (NF) transitional B cells that migrate from the bone marrow, without changes in other immune or stromal cell compartments. Transient

integrin alpha 6 beta 1-mediated interaction of NF B cells with laminin alpha 5 in the MZ supports the MZ B-cell population, their long-term survival, and antibody response. Data suggest that the unique 3D structure and biochemical composition of the ECM of lymphoid H 89 solubility dmso organs impacts on immune cell fate.”
“Quantification of osteolysis is crucial for monitoring treatment effects in preclinical research and should be based on MicroCT data rather than conventional 2D radiographs to obtain optimal accuracy. However, data assessment is greatly complicated in the case of 3D data. This paper presents an automated method to follow osteolytic lesions quantitatively and visually over time in whole-body MicroCT data of mice.\n\nThis novel approach is based on a previously published approach to coarsely locate user-defined structures of interest in the data and present them in a standardized manner (Baiker et al., Med Image Anal 14:723-737, 2010; Kok et al., IEEE Trand Vis Comput Graph 16:1396-1404, 2010). Here, we extend this framework by presenting a highly accurate way to automatically measure the volumes of individual bones and demonstrate the technique by following the effect of osteolysis in the tibia of a mouse over time. Besides presenting quantitative results, we also give a visualization of the measured volume to be able to investigate the performance of the method qualitatively.