The data are presented as mean ± standard deviation of three determinations. Statistical analyses were performed using a one-way analysis of variance. Results were calculated by employing the statistical software (SPSS). Data are expressed as mean ± standard deviation (n = 3). P values: P < 0.05 (a); P < 0.01 (b); P < 0.001 (c) compared to the control value, respectively. n-Hexacosane (1): mp 56–58 °C,11 white solid, C26H54,m/z 366 (M+), IR (vmax) cm−1 (KBr): 2940, 2880, 730, 720. Polypodatetraene (2): pale yellow oil,12 C30H50, m/z 410
(M+), IR (vmax) cm−1 (KBr): 1650, 1630, 1385, 1370, 890.1H NMR (CDCl3, 300 MHz): 5.12 (3H, t), 2.01–1.15 (38H, m), 0.88 (3H, s), 0.85 (3H, s) and 0.82 (3H, ZD6474 nmr s). α-Amyrin acetate (3): mp 222–223 °C,13 and 14 white needles, C32H52O2, m/z 468 (M+), IR (vmax) cm−1 (KBr): 1730, 1650, 1380, 1350, 1250. 1H NMR (CDCl3, 300 MHz): 5.12 (1H, t), 4.50 (1H, dd), 2.05 (3H, s), 1.93-1.13 (23H, m), 1.06–0.78 (8 × CH3). Gluanol acetate (4): mp 184–85 °C,14 white needles, C32H52O2, m/z 468 (M+), IR (vmax) cm−1 (KBr):
1740, 1640, 1380, 1350, 1240, 970, 820. 1H NMR (CDCl3, 300 MHz): 5.18 (2H, m), 4.50 (1H, m), 2.05 (3H, s), 1.98–1.13 (22H, m) and 1.06-0.79 (9 × CH3). 13C NMR (CDCl3, 75 MHz): 171.0 (C O, C-1′), 145.2 (C-8), 139.7 (C-9), 124.3 (C-22), 121.6 (C-33), 80.9 (C-3), 59.0 (C-17), 55.2 (C-14), 47.5 (C-5), 41.6 (C-20), 39.7 (C-13), 37.7 (C-4), 34.7 (C-10), 33.3 (C-25), 39.6–25.9 (9 × CH2), HIF inhibitor 23.5–15.5 (9 × CH3). Lupeol acetate (5): mp 278–80 °C,15 white needles, C32H52O2, m/z 468 (M+), IR (vmax) cm−1 (KBr): 1750, 1640, 1385, 1360, 1310, 1245, 880. 1H NMR (CDCl3, 300 MHz): Cell press 4.69
(1H, broad s), 4.57 (1H, broad s), 4.40 (1H, m), 2.37 (1H, m), 2.04 (3H, s), 1.68 (3H, s), 1.64-1.20 (24H, m), 1.04 (3H, s), 0.97 (3H, s), 0.87 (3H, s), 0.85 (3H, s), 0.83 (3H, s), 0.78 (3H, s). β-Amyrin acetate (6): mp 236–37 °C,14 white powder, C32H52O2, m/z 468 (M+), IR (vmax) cm−1 (KBr): 1730, 1650, 1380, 1360, 1250, 960, 820. 1H NMR (CDCl3, 300 MHz): 5.12 (1H, t), 4.50 (1H, dd), 2.05 (3H, s), 1.93-1.13 (23H, m), 1.06–0.78 (8 × CH3). Bergenin (7): mp 236–38 °C,16 and 17 white granules, C14H16O9, m/z 328 (M+), IR (vmax) cm−1 (KBr): 3400 (broad) 1705, 1620, 1250, 1180, 1125, 1040, 1020, 990, 760. 1H NMR (CDCl3, 300 MHz): 7.58 (s, H-7), 4.85 (d,J = 10.2 Hz), 4.06 (dd, J = 12.3, 9.6 Hz), 3.99 (d, J = 6.0 Hz), 3.91 (3H, s, H-12), 3.85 (dd, J = 9.3, 8.7 Hz), 3.70 (1H, m, H-2), 3.49 (1H, t, J = 9.3 Hz).