Survival signals to CD8+ T cells by up-regulating cellular FLIPs, followed by inhibiting caspase activation were previously identified [35]. This was also observed in reduced rTNF-related apoptosis after treatment of CD8+ cells with antigenic fractions. After exposure to rTNF-α, CD8+ T cells effectively survived when they were re-exposed to H. polygyrus antigen. The influence of GITR stimulation on CD8+ T cells and the nature of parasitic nematode antigens have yet to be determined. Heligmosomoides polygyrus antigens supported survival of CD8+ cells also when apoptosis was induced by TNF receptor. TNF-α maintains lymphocyte number by modulation of Selleck PS341 their apoptotic death

programme and synthesis of pro- and antiapoptotic proteins depending on the presence of active transcription factors, such as NF-κB [36]. The difference in sensitivity to rTNF-α-induced apoptosis between cell populations in this study was evident. The most sensitive population comprised CD4+CD25hi T cells and high level of apoptosis was

preferentially expressed by these cells when they were treated with rTNF-α; almost 50% of these cells undergo apoptosis. Although Th2 response is typical for H. polygyrus infection, TNF-α production temporary increased on day 12 [24]. Interestingly, both naïve and restimulated CD4+CD25hi cells preferentially expressed Bcl-2. Costimulation via TNF-α receptor and TCR with rTNF-α and with H. polygyrus antigens, Silmitasertib mouse respectively, did not change the percentage of apoptotic cells, with the exception of F13

which discriminated between naïve and activated cells. Fraction 17 slightly supported survival of both naïve and activated cells; it may rather regulate Bcl-2 expression by CD4+CD25hi cells when they were exposed to that fraction. The better survival of Treg cells is dependent on Bcl-2 protein [37], and factors which support these cells surviving might Carnitine palmitoyltransferase II be present in F17. After restimulation, the same fraction also inhibited apoptosis of CD4+ T cells. The inflammatory effects of TNF-α are mediated by signalling through the type I (TNFRI) or type II (TNFRII) receptors. Induction of TNF receptor I (TNFR1) signalling is known to activate the transcription factor NF-κB and promote survival of cells [38]. Only in response to complete antigen and to F9, activity of NF-κB p50 subunit was enhanced and selective for the restimulated cells. It is also likely that factors that are present in F9 regulated the number or abundance of Treg cells via TNFR2. TNFR2 is preferentially expressed by highly functional mouse Treg cells and mediates the activating effect of TNF-α on Treg cells [39, 40]. The different recognition of TNF alpha receptor types could help identify the nematode factors involved in the regulation of Treg response and needs further studies.