Table 2 Afa/Dr adhesins distribution in DAEC strains isolated from cases of
diarrhea and asymptomatic controls Strains isolated from Children Adults Diarrhea Control Diarrhea Control afaE N (%) N (%) Total N (%) N (%) Total 1 22 (44) 19 (32.8) 41 (38) 12 {Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|buy Anti-infection Compound Library|Anti-infection Compound Library ic50|Anti-infection Compound Library price|Anti-infection Compound Library cost|Anti-infection Compound Library solubility dmso|Anti-infection Compound Library purchase|Anti-infection Compound Library manufacturer|Anti-infection Compound Library research buy|Anti-infection Compound Library order|Anti-infection Compound Library mouse|Anti-infection Compound Library chemical structure|Anti-infection Compound Library mw|Anti-infection Compound Library molecular weight|Anti-infection Compound Library datasheet|Anti-infection Compound Library supplier|Anti-infection Compound Library in vitro|Anti-infection Compound Library cell line|Anti-infection Compound Library concentration|Anti-infection Compound Library nmr|Anti-infection Compound Library in vivo|Anti-infection Compound Library clinical trial|Anti-infection Compound Library cell assay|Anti-infection Compound Library screening|Anti-infection Compound Library high throughput|buy Antiinfection Compound Library|Antiinfection Compound Library ic50|Antiinfection Compound Library price|Antiinfection Compound Library cost|Antiinfection Compound Library solubility dmso|Antiinfection Compound Library purchase|Antiinfection Compound Library manufacturer|Antiinfection Compound Library research buy|Antiinfection Compound Library order|Antiinfection Compound Library chemical structure|Antiinfection Compound Library datasheet|Antiinfection Compound Library supplier|Antiinfection Compound Library in vitro|Antiinfection Compound Library cell line|Antiinfection Compound Library concentration|Antiinfection Compound Library clinical trial|Antiinfection Compound Library cell assay|Antiinfection Compound Library screening|Antiinfection Compound Library high throughput|Anti-infection Compound high throughput screening| (44.4) 5 (33.3) 17 (40.5) 2 5 (10) 3 (5.2) 8 (7.4) 1 (3.7) 2 (13.3) 3(7.1) 3 1 (2) 1 (1.7) 2 (1.8) 2 (7.4) 1 (6.7) 3 (7.1) 5 1 (2) 2 (3.5) 3 (2.8) 1 (3.7)a 7 (46.7)a 8 (19) X 12 (24) 7 (12) 19 (17.6) 11 (40.7)a 0a 11 (40.7) daaE 3 (6) 9 (15.5) 12 (11) 0 0 0 1 + 2 5 (10) 0 5 (4.6) 0 0 0 1 + 3 0 1 (1.7) 1 (0.9) 0 0 0 1 + 5 0 6 (10.3) 6 (5.1) 0 0 0 1 + daaE 1 (2) 5 (8.6) 6 (5.1) 0 0 0 1 + 2 + daaE 0 1 (1.7) 1 (0.9) 0 0 0 2 + daaE 0 2 (3.5) 2 (1.8) 0 0 0 5 + daaE 0 2 (3.5) 2 (1.8) 0 0 0 Total 50 58 108 27 15 42
aP < 0.05 (cases x control). In 20% (30/150) of afaB-C-positive strains, the adhesin gene could not be identified. These strains with indeterminate afaE were referred to as “afa-X”. Strains isolated from children and adults exhibited see more a very different distribution of Afa/Dr adhesin encoding genes. The afaE-1 gene was a notable exception, being similarly distributed for all groups. It was also the most frequent gene. Strains isolated from children showed great diversity of adhesins. More than one type of Afa/Dr adhesins were detected in 21.3% (23/108) of strains isolated from children, and in 29.3% (17/58) of strains isolated from asymptomatic children. All genetic combinations involve afaE-1 or daaE. The afaE-1/afaE-2 association was found only in diarrheagenic strains (P < 0.05). The F1845 encoding gene was only found in strains isolated from Oxymatrine children, especially in control strains. Strains isolated from adults showed a low variability of afaE genes.
Prevalence of afa-X was higher (P < 0.01) in cases of diarrhea, while prevalence of afaE-5 was higher in controls (P < 0.01). Neither the daaE gene nor associations between two types of adhesins were detected in strains from adults. Distribution of virulence factors DAEC strains were examined regarding characteristics associated with virulence. The percentage of strains carrying virulence genes or possessing phenotypic characteristics associated to biofilm formation is summarized in Table 3. Table 3 Characteristics associated to virulence in DAEC strains possessing Afa/Dr genes isolated from children and adults Strains isolated from N(%) Children Adults Characteristic Diarrhea Control Diarrhea Control traA 45 (90) 47 (81) 19 (70.3) 13 (86.6) Cellulose 5 (10) 17 (29.3) 1 (3.7) 0 Curli 31 (62) 39 (67.2) 16 (59.2)a 1 (6.7)a sat 23 (46) 11 (18.9) 18 (66.7) 13 (86.6) TTSS 3 (6) 30 (51.