The filtrate was used for the preliminary phytochemical analysis. The tests were performed according to methods described by Khandelwal (1998) and Kokate (2007). 12 and 13 TLC for various phytoconstituents was carried out as per methods described by Wagner and Bladt (1996).14 Albino Wistar rats, 8–12 weeks old, weighing in range of 120–180 g, was procured from Haffkine Institute, Parel. The animals were accommodated Selleckchem EPZ6438 in groups of five in polypropylene cages with stainless steel grill
top and a bedding of clean paddy husk was provided. The animals were maintained in air conditioned room with controlled temperature maintained in the range of 22–25 °C and alternating 12 h periods of light and dark cycle. The relative humidity was close to 60%. The animals were acclimatized to standard laboratory conditions prior to experimentation. The guidelines issued by Institutional Animal Ethics Committee of Ramnarain Ruia College, Mumbai, India with CPC SEA registration GSK1210151A no. CPC SEA/315, regarding the maintenance and dissection of small animals were strictly followed. Rats were administered a dose of 2000 mg/kg body weight for 14 days and were then examined for any signs of behavioural changes and mortality. All experiments were performed on female Albino Wistar rats (200–250 g)
obtained from the Haffkine Institute, Parel, Mumbai, Maharashtra, India. The animals were accommodated in groups of six in polypropylene
cages with stainless steel grill top and a bedding of clean paddy husk. Animals were maintained under a constant 12-h period of light and dark cycle and an environmental temperature of 22–25 °C. The Tryptophan synthase animals were acclimatized for 15 days before being used for the experiments. The guidelines issued by Institutional Animal Ethics Committee of Ramnarain Ruia College, Mumbai, India with CPC SEA registration no. CPC SEA/315, regarding the maintenance and dissection of small animals were strictly followed. The animals were fed on the standard pellet diet (Amrut Feed, Pune) and water was given ad libitum. The overnight fasted rats were made diabetic with streptozotocin (STZ) (Sigma, St Louis, MO; 60 mg/kg; intraperitoneally). The STZ was prepared freshly by dissolving it in Na-citrate buffer (0.01 M, pH 4.5) and maintained on ice prior to use; the injection volume was 0.2 ml. Diabetes was confirmed in the rats by measuring the fasting blood glucose concentration after 72 h of STZ administration. The rats with glucose level above 300 mg/dl were considered to be diabetic and were used in the experiment. Animals had free access to food and water after the STZ injection.