Thirteen isolates were assigned to species level with low demarcation to the next species but supplemental conventional tests revealed a final identification to species APR-246 chemical structure level (Table 1). Conventional methods assigned 60% of the isolates to species level and 15% to genus level (Tables 1 and 2). However, only 40% were correctly assigned to species level and 13% correct to genus level considering the 16S rRNA gene sequencing as reference method. 47% of the isolates were misidentified or not identified

by conventional methods; nevertheless, 18 of the 31 isolates incorrectly assigned to species level were identified to the correct genus (Table 2). Table 1 Identification of clinical isolates (n=158) by conventional methods compared to 16S rRNA gene sequence analysis Conventional phenotyic methods   16S rRNA gene sequence analysis     Final identification (supplemental conventional tests if required) selleck Identification (number of isolates) Level of identification and correctness of result Best reference species sequence % difference to reference species sequence GenBank accession numbers   Actinobacillus ureae (1) S 1; SI 2 Actinobacillus hominis Actinobacillus suis (low demarcation) 0.0, 0.4 KC866152 A. hominis (acidification of mannitol: A. hominis (positive), A. suis (negative) [1]) Aggregatibacter actinomycetemcomitans (2) S; SC Aggregatibacter actinomycetemcomitans 0.0, 0.3 KC866227; KC866228 A. actinomycetemcomitans

Aggregatibacter actinomycetemcomitans (1) S; SI Pasteurella bettyae 0.0 KC866143 P. bettyae Aggregatibacter aphrophilus (11) S; SC Aggregatibacter aphrophilus 0.0-0.8 KC866144; KC866145; KC866146; KC866147; KC866148; KC866149; KC866150; KC866229; KC866230; KC866231; KC866272 A. aphrophilus Aggregatibacter aphrophilus (2) during S; SI Aggregatibacter aphrophilus 3.8, 2.9 KC866151; KC866153 Aggregatibacter sp. Aggregatibacter aphrophilus (1) S; SI Neisseria sicca 0.8 KC866154 N. sicca (nitrate reduction: positive (N. mucosa), negative (N. sicca, N. subflava bv. flava); sucrose acidification: positive (N. sicca, N. mucosa),

negative (N. subflava bv. flava) [18]) Neisseria subflava bv. flava 1.0 Neisseria mucosa (low demarcation) 1.1 Aggregatibacter sp. (1) G; GC Aggregatibacter aphrophilus 2.3 KC866155 Aggregatibacter sp. Topoisomerase inhibitor Bergeyella zoohelcum (1) S; SI Myroides odoratimimus 5.9 KC866156 Flavobacteriaceae Bergeyella zoohelcum (1) S; SI Neisseria zoodegmatis 0.3 KC866157 N. zoodegmatis Capnocytophaga canimorsus (2) S; SC Capnocytophaga canimorsus 0.5, 0.4 KC866158; KC866159 C. canimorsus Capnocytophaga ochracea (1) S; SI Capnocytophaga gingivalis 0.6 KC866160 C. gingivalis Capnocytophaga ochracea (1) S; SI Capnocytophaga ochracea 2.5 KC866161 Capnocytophaga sp. Capnocytophaga ochracea (5) S; SI Capnocytophaga sputigena 0.0-0.3 KC866162; KC866163; KC866164; KC866273; KC866274 C. sputigena 3 Capnocytophaga ochracea (1) S; SI Dysgonomonas mossii 0.6 KC866165 D. mossii Capnocytophaga ochracea (1) S; SI Leptotrichia trevisanii 0.