Typhi to produce a systemic infection in humans. sopD2 gene corresponds to an SPI-2-regulated effector protein (Brumell et al., 2003). In S. Typhi this gene carries a nucleotide deletion that produced a nonsense mutation and probably Protein Tyrosine Kinase inhibitor the loss of an essential protein translocation motif [WEK(I/M)xxFF; data not shown] (Brumell et al., 2003; Brown et al., 2006). Our results confirm that sopD2 in S. Typhi is a pseudogene supporting previous studies of Salmonella spp. comparative genomics (Parkhill et al., 2001; McClelland et al., 2004). The SPI-1 encodes T3SS effectors that mediate the invasion by Salmonella of nonphagocytic cells via cellular host cytoskeleton manipulation (Bueno et al., 2010). SPI-2

is induced after bacterial internalization and is essential for bacterial survival and proliferation within SCV. In addition, intracellular Salmonella interferes with the actin cytoskeleton in an SPI-1 T3SS-independent manner and in SPI-1-dependent effectors contributing to the SCV establishment (Abrahams & Hensel, 2006; Bakowski et al., 2008). This has been described as a regulatory and functional SPI cross-talk (Knodler et al., 2002; Steele-Mortimer et al., 2002). It is therefore not surprising that although sopD2 in S. Typhimurium corresponds to a SPI-2-regulated

effector, PI3K Inhibitor Library it is also expressed under SPI-1 conditions and participates in epithelial cell invasion (Brumell et al., 2003). The previous observation is supported by S. Typhimurium ΔsopD2∷FRT null mutant strain (NT060), which Flucloronide showed a decreased invasion level in HEp-2 human epithelial cell line. In S. Typhimurium, SCV synthesis depends on SopD2 and the sopD2 gene mutation decreases intracellular proliferation and bacterial virulence in mice (Jiang et al., 2004; Birmingham et al., 2005). However, the presence of a fully functional sopD2 gene interferes with

S. Typhi proliferation within human epithelial cells and the bacterial capacity to alter cellular permeability. Because S. Typhimurium SopD2 participates in the endosome (SCV) synthesis and concomitantly in the generation of Sif structures (Brumell et al., 2003; Jiang et al., 2004; Birmingham et al., 2005), we suggest that SopD2STM in S. Typhi interferes directly in the intracellular traffic of this pathogen in epithelial cells. Our previous studies showed that this permeability alteration is directly related to cytotoxicity (Trombert et al., 2010). In this work, we observed that the functional transfer of sopD2 from S. Typhimurium to S. Typhi decreased cellular permeability and more likely decreased cytotoxicity. It has been observed that S. Typhi increases cytotoxicity within the host by inactivating or acquiring new genes (Oscarsson et al., 2002; Fuentes et al., 2008; Faucher et al., 2009; Trombert et al., 2010). Thus, the inactivation of some genes (i.e. sopD2) and the acquisition of others could contribute to cytotoxicity toward the epithelial barrier and might ensure the development of systemic infection in the human host.