AFLP was applied to our entire “”psilosis”" collection (n = 650), as this method has been shown to reproducibly and unequivocally identify Candida species [16, 17, 19]. The 62 selected isolates were analysed further by using another enzyme/primer combination EcoRI-HindIII, since the previously used EcoRI-MseI combination was found to be less discriminative and affected by band homoplasy in C. parapsilosis and C. metapsilosis [unpublished data, [17]]. The EcoRI/HindIII enzyme combination gives rise to larger fragments and therefore increases the sensitivity

Baf-A1 manufacturer to detect polymorphisms. In parallel, phenotypic properties such as biofilm formation and proteinase secretion were analysed. Since the “”psilosis”" species have been recently associated with a lower susceptibility to the echinocandin class of antifungals [20, 21], drug susceptibility was also evaluated and extended to other antifungals. The overall goal of this study was to gain further information on genotypic and phenotypic properties of this successful and yet elusive opportunistic pathogen. Methods Isolates VX-680 ic50 The Candida parapsilosis collection included 62 individual isolates obtained from check details different body sites and geographical regions (Table 1). The majority of Italian isolates (n = 19) was provided by the Unità Operativa di Microbiologia, Ospedale Universitario, Pisa; 6 isolates being from different Italian

hospitals (Table 1). Hungarian isolates (n = 14) were from the Department medroxyprogesterone of Microbiology, Medical School, Debrecen. Argentinian and New Zealand isolates were kindly provided by Dr Marisa Biasoli, Centro de Referencia de Micologia, University of Rosario and by Dr Arlo Upton, Auckland City Hospital, respectively. The isolates used in this study were initially identified as C. parapsilosis according to their biochemical profile on API32 ID and a Vitek 2 advanced colorimetric semi automated system (bioMérieux, Marcy l’Etoile, France). C. parapsilosis ATCC 22019 was included in the study as reference

strain. All isolates were maintained on Sabouraud agar (Liofilchem S.r.l., TE, Italy) for the duration of the study. Table 1 Details and phenotypic properties of Candida parapsilosis clinical isolates used in this study. Strain Site of isolation Origin Biofilme 30°C Proteasef 30°C CP 1 Conjunctiva Pisa (I) 0.006 (NPi) 0.3 (NP) CP 17 Blood Pisa (I) 0.015 (NP) 1.13 (WP) CP 24 Blood Pisa (I) 0.003 (NP) 3.0 (MP) CP 28 Nail Pisa (I) 0.006 (NP) 1.5 (WP) CP 39 Blood Pisa (I) 0.010 (NP) 1.0 (WP) CP 42 Blood Pisa (I) 0.042 (WPl) 0.5 (NP) CP 66 Vaginal swab Pisa (I) 0.001 (NP) 1.0 (WP) CP 71 Vaginal swab Pisa (I) 0.031 (WP) 1.0 (WP) CP147a Catether Novara (I) 0.031 (WP) 0.3 (NP) CP164a Catether Bergamo (I) 0.024 (NP) 3.5 (HP) CP183a Blood Pavia (I) 0.012 (NP) 5.7 (HP) CP 191a Blood Catania (I) 0.039 (WP) 1.25 (WP) CP 192a Blood Catania (I) 0.034 (WP) 1.