Soil samples for the chemical analysis were cored through the top 20 cm at five randomly selected points in each plot using an Oakfield soil sampler, Fond du Lac, WI. These samples were air dried, passed through a 2-mm sieve, and used for the soil chemical analyses. Soil pH (1:5 soil:water suspension) was measured using a glass electrode. The Nintedanib manufacturer carbon (C) and nitrogen (N) content in the soil were determined using an elemental analyzer (CE Instruments EA1110, Thermo Quest Italia S.P.A., Radano, Italy). Available phosphorus (P), calcium (Ca), magnesium (Mg), and potassium (K) were determined by inductively coupled plasma (Perkin Elmer Optima 5300, Waltham, MA, USA) using the standard method

recommended by the National Institute of Agricultural Science and Technology [8]. The data were LY294002 analyzed

using the general linear model procedure using SAS version 9.1 (SAS Institute Inc., Cary, NC, USA) to determine the significant difference (p < 0.05) of cultivation sites by stand site types and by elevation. The treatment means were compared using Duncan's test [9]. Mountain-cultivated ginseng was cultivated in three natural and three artificial forests with six different overstory stand types: deciduous broad-leaved forests with Carpinus laxiflora, Quercus spp., Acer mono, Prunus sargentii; Cornus controversa: thirteen plots; P. densiflora: eight plots; mixed forests of P. densiflora and Quercus spp.: three plots; L. leptolepis plantation: four plots; Chamaecyparis obtuse plantation: one plot; and Pinus koraiensis plantation: one plot ( Table 1). The soil bulk density was significantly higher for the P. densiflora stand sites (0.96 g/cm3) than for the L. leptolepis stand sites (0.69 g/cm3). Among the three phases of the soil, there was a significantly higher

proportion of the liquid phase for the deciduous broad-leaved (34.0%) and mixed stand sites (34.6%) than for the P. densiflora stand sites (18.8%), but the air phase was Fossariinae reversely related to the liquid phase ( Fig. 1). The soil pH was not significantly different among stand sites, although the soil pH in the mixed stand sites was 0.1–0.2 units higher compared with that of the other stand sites. The soil pH was highest on average in the mixed stand sites (pH 4.55), followed by a pH 4.46 for the P. densiflora stand sites, pH 4.36 for the deciduous broad-leaved stand sites, and pH 4.35 for the L. leptolepis stand sites ( Fig. 2). All of the stands were strongly acidified, with a soil pH below 4.55. The organic C and total N content were significantly higher for the deciduous broad-leaved stand sites (C: 6.16%; N: 0.44%) than for the P. densiflora (C: 2.64%; N: 0.19%) stand sites. The C/N ratio ranged from 12.8 to 16.5, with the highest value of 16.5 in P. densiflora stand sites. The available P was low in all of the stand sites.