The study protocol was approved by the ethics committee of the Hospital Clinic of Barcelona. On June 19, 2009, the medical students traveled from Barcelona to Santo Domingo, Dominican Republic on a scheduled flight with a stopover in Madrid. A bus took them to their hotel located at a beach resort in Punta Cana. Meals were shared depending on daily activities organized and the number of students participating in each activity. The students slept in rooms for two, three,

or four Dabrafenib nmr people with members of their own travel group. Activities were organized according to the interests of the students, and included sightseeing excursions. On June 26, all students traveled on the same bus on a 4-hour trip from Punta Cana to Santo Domingo, where they boarded an Airbus aircraft with 284 economy class and 20 business class seats. The flight back

to Spain lasted 8 hours. Of the 113 students, 86 (76%) were contacted and agreed to participate in the study. The rest could not be contacted or declined to participate. Of the 86 students, 58 (67%) were female. The median age was 24 years, (range 22–56 y). A total of 62 (72%) students developed ILI, and influenza A(H1N1) was confirmed in 39 (45%) (two confirmed cases GSK2126458 were asymptomatic). Thus, assuming that none of the students who did not participate were ill or infected, the minimum attack rate among all 113 students was 55% for probable influenza and 35% for confirmed influenza. Two of the 37 confirmed cases developed symptoms during the stay in the Dominican Republic. The

first confirmed case first developed symptoms on June 24, followed by a second case on June 25 (2 and 1 d before starting the return trip, respectively). Between June 26 (day of departure ADAMTS5 from Santo Domingo) and 48 hours after arriving in Barcelona, 29/39 (74%) of the students with confirmed A(H1N1) infection developed symptoms; 6 students (15%) developed symptoms more than 72 hours later, and 2 remained asymptomatic (Figure 1). The predominant symptoms in confirmed cases (Table 1) were cough (87%), malaise (60%), and sore throat (51%). Gastrointestinal symptoms (diarrhea) were reported by 16 (43%) of the confirmed cases. Univariate analyses showed that cough, fever, myalgia, rhinorrhea, and malaise were significantly associated with confirmed infections, and this was supported by the logistic regression analysis (Table 1). Laboratory testing for influenza was more likely to be negative when the time between the onset of illness and the day of diagnostic sampling was longer (Figure 2). The mean time between onset of symptoms and blood sampling was 3.5 days; most (92%) of the positive samples were obtained between 1 and 3 days after onset, whereas most (83%) of the negative samples were obtained 3 or more days after onset. On arriving home from the trip, the students went to their homes, where they lived with family or other students.