Collectively, these experiments highlight the necessity and function of multiple related, cytoplasmic host sensors in orchestrating an effective immune response against an acute viral infection.”
“The antiproliferative immunosuppressive drug mycophenolic acid (MPA) is an uncompetitive inhibitor of inosine monophosphate dehydrogenase, a key enzyme in de novo synthesis of purine nucleotides. The latter are not only required for synthesis of DNA and RNA but also are essential for the regulation of numerous cellular signaling pathways modulated by guanine nucleotide binding proteins (G proteins). We undertook an analysis of

the influence of MPA on protein expression in a T-lymphoblast cell line (CCRF-CEM): which displays concentration-dependent inhibition of proliferation by MPA to Autophagy Compound Library obtain insight into the influence of MPA on the cellular proteome. Cells were stimulated with phorbol myristate acetate/ionomycin and incubated in the presence

or absence of MPA. Two-dimensional electrophoresis and densitometric imaging revealed 11 differentially expressed protein spots (P < 0.05) on MPA treatment. 6 with increased and 5 with decreased abundance. After HIF inhibitor in-gel tryptic digestion, proteins were identified by quadrupole time-of-flight mass spectrometry. Proteins displaying increased abundance after MPA treatment included splicing factor arginine/serine-rich 2, prostaglandin E synthase 3. peptidyl-prolyl cis-trans isomerase A, and deoxyuridine 5′-triphosphate nucleotidohydrolase. Endoplasmin, proliferating cell nuclear antigen, acidic Compound Library research buy leucine-rich nuclear phosphoprotein 32 family member A, and cofilin I showed decreased abundance after MPA treatment. Three separate spots (I decreased and 2 increased abundance) were identified as Rho guanosine diphosphate dissociation inhibitor 2 (Rho GDI 2) proteins. Western blotting with a monoclonal antibody directed against the Rho GDI 2 site cleaved by caspase 3 demonstrated I spot with increased abundance to be the caspase 3-cleaved product of Rho GDI 2 lacking the first 19 amino acids. Rho GDI 2 plays a central

regulatory role in the activation of Rho guanosine triphosphatases that function as molecular switches in cell signaling pathways affecting cell cytoskeletal dynamics and motility. Our data suggest that MPA can modulate Rho GDI 2 levels in T lymphocytes, thereby potentially disrupting cell signaling pathways important for T-cell function.”
“Antioxidant and radical scavenging properties of a series of 2-[4-(substituted piperazin-/piperidin-1-ylcarbonyl)phenyl]-1H-benzimidazole derivatives were examined. Free radical scavenging properties of compounds 11-30 and 33 were evaluated for the stable free radical 2,2-diphenyl-1 -picrylhydrazyl (DPPH) and superoxide anion radical. In addition the inhibitory effects on the NADPH-dependent lipid peroxidation levels were determined by measuring the formation of 2-thiobarbituric acid reactive substances (TBARS) using rat liver microsomes.

Phenol-formaldehyde mixtures were impossible to detoxify by heterogeneous photocatalysis at any of the studied concentrations. Treatments using the Fenton reaction were able to degrade concentrations above 1000 mg L-1, though the use of a reagent such as peroxide makes it a costly technique. The efficiency of the biological aerated filter (BAF) mainly depended on initial concentration and toxicity, with

removal rates of 3.08 and 0.26 g L-1 d(-1) obtained for phenol and formaldehyde, respectively. Taking into account the results obtained for the treatment of complex phenol-formaldehyde mixtures, the best combination of techniques for the treatment of concentrations found in the industrial wastewater studied in this MCC950 inhibitor paper was the Fenton + BAF technique which was able to detoxify phenol-formaldehyde

concentrations (1:1) of 1000 mg L-1. (C) 2014 Elsevier B.V. All rights reserved.”
“We present Fourier-transform infrared (FTIR) spectroscopic studies of the IR-induced CI + H-2 (v = 1) – bigger than HC1 + H reaction in a parahydrogen (pH(2)) matrix aimed at distinguishing between two proposed reactions mechanisms; direct-IR and vibron-mediated. The Cl atom reactants are produced via 355 nm in situ photolysis of a Cl-2 doped pH(2) matrix. After photolysis is complete, a long-pass IR filter in the FTIR beam is removed and we click here measure the ensuing IR-induced reaction kinetics using rapid scan FTIR spectroscopy. We follow both the decay of the Cl atom reactant and growth of the HCl product using the Cl spin-orbit (SO) + Q(1)(0) and HC1 R-1(0) transitions, respectively. We show the IR-induced reaction mechanism depends on the spectral profile of the IR radiation; for IR spectral profiles that have significant IR intensities between 4000 and 5000 cm(-1) we observe first-order kinetics that are assigned to a vibron-mediated mechanism and for spectral profiles that have significant IR intensities that include the Cl SO + Q1(0) transition near 5094 cm(-1) we observe bi-exponential kinetics that are dominated by the direct-IR mechanism at early reaction times. We can distinguish between the two

mechanisms using the observed kinetics. We investigate the reaction kinetics for different FTIR optical setups, for a range of sample SNX-5422 cell line conditions, and start and stop the IR-induced reaction to investigate the importance of secondary H atom reactions. We also study the IR-induced reaction in Br/Cl co-doped pH2 samples and show the presence of the Br atom quenches the vibron-mediated reaction kinetics presumably because the Bratoms serve as efficient vibron traps. This paper indicates that in a highly enriched pH2 matrix the H atoms that are produced by the IR-induced Cl atom reaction likely do not play a significant role in the measured reaction kinetics which implies these secondary H atom reactions are highly selective. (C) 2015 Elsevier Inc. All rights reserved.

The EDM tendon was found to be bifurcated

in 74% (n = 36) of hands and all of these SB203580 in vitro hands contained a synovial septum. In 9 (25%) hands, the EDM tendon bifurcated proximal to the retinaculum, in 15 (42%), it bifurcated distal to the retinaculum, and in the other 12 hands (33%), the tendon bifurcated at the retinacular level. In 6 of the 15 hands with an infraretinacular bifurcation, the tendon was found to impinge on the synovial septum during passive flexion of the wrist with full finger flexion, and the mean distance between the synovial septum and the bifurcation point in these specimens was 0.6 cm (range, 0.40.7 cm), which was differed significantly from hands not showing impingement (P = 0.01). This study shows that distal bifurcation of the EDM selleck compound tendon may lead to tendon impingement on the septum and suggests that this is a potential etiology of chronic tenosynovitis of the fifth compartment and of acute closed tendon injuries. Clin. Anat. 25:755761,

2012. (C) 2011 Wiley Periodicals, Inc.”
“Despite intensive control efforts over the past decades, Brazil still accounts for more than 50% of the malaria burden in the Americas and the Caribbean, with 458,041 slide-confirmed cases reported countrywide in 2007. The reason malaria has proved so difficult to control in this middle-income country with a reasonable health infrastructure remains unclear. Here we examine whether four strategies that were largely successful in other countries (aggressive active case detection, improved anti-relapse therapy for P. vivax infections, distribution of insecticide-treated bed nets, and selective Rabusertib house spraying with residual insecticides) are likely to work in Brazil. We review evidence from field and laboratory studies and identify gaps in our knowledge that require further investigation with well-designed large-scale trials.”
“Objectives: In this pilot study we evaluated

the feasibility of and methods for assessing the quality of life of long term survivors of European Organisation for Research and Treatment of Cancer (EORTC) phase III clinical trials. Here we report the results pertaining to the feasibility of conducting such research. Methods: In this cross-sectional study, we recruited long-term, disease-free survivors from two mature EORTC clinical trials in testicular and prostate cancer from centres in Northern and Southern Europe, and the United Kingdom (UK). Results: A number of challenges were encountered in recruiting participating centres, obtaining medical ethical approval and in recruiting survivors and collecting the health-related quality of life (HRQoL) data in a timely manner. The efficiency with which the study could be conducted varied widely across centres and countries. Time to obtain medical ethical approval for the study ranged from 1.5 to 25 months.

As Etv5 has been suggested to influence dopaminergic neurotransmission by driving the expression of genes that

are responsible for the synthesis and release of dopamine, we investigated if expression levels of Etv5 are dependent on nutritional state and subsequently influence the expression levels of tyrosine hydroxylase. While it was shown that Etv5 expression in the VTA/SNpc increases after central administration of leptin and that Etv5 was able to drive expression of tyrosine hydroxylase in vitro, AAVmediated gene transfer of Etv5 into the VTA/SNpc of rats did not alter expression of tyrosine hydroxylase in vivo. Moreover, AAV-mediated gene transfer of Etv5 in the VTA/SNpc did not affect measures of energy balance or performances in a progressive ratio schedule. Thus, these data do not support AZD4547 inhibitor a role for increased expression of Etv5 in the VTA/SNpc in the regulation of feeding behavior.”
“Background: Cancer chemoprevention is considered one of the most promising areas in current cancer research, and asiaticoside, which is derived from the plant Centella asiatica, has a relative lack of systemic toxicity. The purpose of this study was to investigate whether asiaticoside is effective against 7,12-dimethylbenz(a)anthracene (DMBA)-induced carcinogenicity in vitro (MCF-7 and other cells) and in vivo (DMBA-induced rat cancer). Methods: An MTT assay was performed involving the treatment of MCF-7 cells

for 48 h with H2O2 alone and H2O2 + different

asiaticoside concentrations. Flow cytometry was performed, and the level of caspase 3, tumour selleck chemical necrosis factor-alpha (TNF-alpha) and interleukin-1 (IL-1) were quantified. Adult female Sprague-Dawley (SD) rats were divided into five groups designated I (control), Selleck BLZ945 II (DMBA-induced cancer), III (pre- and post-treatment with asiaticoside (200 mu g/animal) in DMBA-induced cancer), IV (post-treatment with asiaticoside in DMBA-induced cancer), and V (treated with asiaticoside alone, drug control). Twelve weeks post-DMBA, rats developed mammary tumours. Rats either were sacrificed or imaged with MIBI. Histological examination of tumour tissues was performed. Tumour MIBI uptake ratios were determined. The data are expressed as the means +/- standard deviation. Appropriate t-test and ANOVA statistical methods were used to compare data. Results: The IC50 of asiaticoside for MCF-7 cells was determined to be 40 mu M. Asiaticoside has potential for hydrogen peroxide cytotoxicity, and the caspase-3 activity increased with increasing asiaticoside dose in MCF-7 cells treated for 48 h. The expression of the cytokines TNF-alpha and IL-1 beta was significantly decreased and correlated with MIBI uptake ratios in vitro and in vivo after asiaticoside administration. Conclusion: This study demonstrates that asiaticoside is effective in vitro and in vivo in inducing apoptosis and enhancing anti-tumour activity.

Here we identify the Rap1 guanine nucleotide Sulfobutylether-��-Cyclodextrin exchange factor PDZ-GEF2 as an upstream activator of Rap1 required for the maturation of adherens junctions in the lung carcinoma cells A549. Knockdown of PDZ-GEF2 results in the persistence of adhesion zippers at cell-cell contacts. Activation of Rap1A rescues junction maturation in absence of PDZ-GEF2, demonstrating that Rap1A is downstream of PDZ-GEF2

in this process. Moreover, depletion of Rap1A, but not Rap1B, impairs adherens junction maturation. siRNA for PDZ-GEF2 also lowers the levels of E-cadherin, an effect that can be mimicked by Rap1B, but not Rap1A siRNA. Since junctions in Rap1B depleted cells have a mature appearance, these data suggest that PDZ-GEF2 activates Rap1A and Rap1B to perform different functions. Our results present the first direct evidence that PDZ-GEF2 plays a critical role

in the maturation of adherens junctions. (C) 2008 Elsevier Inc. All rights reserved.”
“Among alternative therapeutic strategies in clinically aggressive neuroendocrine tumors (NETs) of the lung, promising results have been obtained in experimental clinical trials with mammalian target of rapamycin (mTOR) inhibitors, though in the absence of a proven mTOR signaling activation Saracatinib order status. This study analyzed the expression of phosphorylated mTOR (p-mTOR) and its major targets, the ribosomal p70S6-kinase (S6K) and the eukaryotic initiation factor 4E-binding protein 1 (4EBP1) in a large series of 218 surgically resected, malignant lung NETs, including 24 metastasizing typical carcinoids, 73 atypical carcinoids, 60 large cell neuroendocrine carcinomas (LCNECs), and 61 small cell carcinomas (SCLCs). By immunohistochemistry, low-to-intermediate-grade tumors as compared with high-grade tumors showed higher levels of p-mTOR and phosphorylated S6K (p-S6K) (P < 0.001), at variance with phosphorylated 4EBP1 (p-4EBP1), which was mainly expressed in

LCNECs and SCLCs (P < 0.001). Syk inhibitor The activated status of mTOR pathway was proved by the strong correlation of p-mTOR with p-S6K and somatostatin receptor(s). Western blot analysis of NET tumor samples confirmed such findings, and differential sensitivity to mTOR inhibition according to mTOR pathway activation characteristics was determined in two lung carcinoid cell lines in vitro. None of the investigated molecules had an impact on survival. However, in low-grade tumors, low p-mTOR expression correlated with lymph node metastases (P = 0.016), recurrent disease, and survival (P = 0.005). In conclusion, these data demonstrate a differential mTOR activation status in the spectrum of pulmonary NETs, possibly suggesting that mTOR pathway profiling might play a predictive role in candidate patients for mTOR-targeted therapies.

In addition, the presence of many microsatellites was a common feature of the 3′-UTR learn more of HSP70-I genes in the Leishmania genus. Finally, we constructed dendrograms based

on global sequence alignments of the analyzed Leishmania species and strains, the results indicated that this particular region of HSP70 genes might be useful for species (or species complex) typing, improving for particular species the discrimination capacity of phylogenetic trees based on HSP70 coding sequences. Given the large size variation of the analyzed region between the Leishmania and Viannia subgenera, direct visualization of the PCR amplification product would allow discrimination between subgenera, and a HaeIII-PCR-RFLP analysis might be used for differentiating some

species within each subgenera.\n\nConclusions: Sequence and phylogenetic analyses indicated that this region, which is readily amplified using a single pair of primers from both Old and New World Leishmania species, might be useful as a molecular marker for species discrimination.”
“The aim of the study was to examine age-related differences in the maximal power and height of rise of the body’s centre of mass, measured in the counter-movement jump (CMJ) and the spike jump (SPJ), between elite cadet, junior and senior female volleyball players. The study was conducted on elite cadet (n=39), junior NVP-AUY922 Cytoskeletal Signaling inhibitor (n=8) and senior (n=23) female volleyball players. The maximal power and height of jumps were measured for CMJ and SPJ. Cadets had a significantly smaller maximal relative power output (40.92+/-8.10 W . kg(-1)) than junior (49.47+/-6.47 W kg(-1)) and senior (46.70+/-8.95 W . kg(-1)) volleyball players during SPJ. The height of rise of the centre of mass measured in CMJ and SPJ were similar between groups. Our research has shown that age-related differences JQ-EZ-05 in vivo were observed only in power output of SPJ. The differences between elite cadet, junior and senior female volleyball

players were not statistically significant in relation to height of jumps (both CMJ and SPJ), and maximal power in CMJ.”
“Immunosenescence, defined as the age-associated dysregulation and dysfunction of the immune system, is characterized by impaired protective immunity and decreased efficacy of vaccines. An increasing number of immunological, clinical and epidemiological studies suggest that persistent Cytomegalovirus (CMV) infection is associated with accelerated aging of the immune system and with several age-related diseases. However, current evidence on whether and how human CMV (HCMV) infection is implicated in immunosenescence and in age-related diseases remains incomplete and many aspects of CMV involvement in immune aging remain controversial.

“
“Honeybee foragers that find a profitable food source quickly establish spatiotemporal learn more memories, which allow them to return to this foraging site on subsequent days. The aim of this study was to investigate how the previous experience

of honeybee foragers at a feeding location affects their persistence at that location once food is no longer available. We hypothesised that persistence would be greater to locations that were more rewarding (closer to the hive, higher concentration of sucrose solution), for which a bee had greater prior experience (0.5-h vs. 2-h training access), and at times of the year of lower nectar availability in the environment. We studied individually marked worker bees from four colonies trained to sucrose-solution feeders. Our results support most of these predictions. Persistence, measured both in duration and number of visits, was greater to locations that previously offered sucrose solution of higher concentration (2m vs. 1m) or were closer to the hive (20m vs. 450m). Persistence was also greater

in bees that had longer access at the feeder before the syrup was terminated (2h vs. 0.5h). However, contrary to our prediction, persistence was not higher in the season of the lowest nectar availability in the environment in the study year. In summary, honeybees show considerable persistence at foraging sites that ceased providing rewards. RSL3 research buy The decision to abandon a foraging site depends on the profitability the forager experienced when the foraging site was still rewarding.”
“Coffin-Siris syndrome (CSS) and Nicolaides-Baraitser syndrome (NCBRS) are rare intellectual disability/congenital malformation syndromes that represent distinct entities but show considerable clinical overlap.

They are caused by mutations in genes encoding members of the BRG1- and BRM-associated factor (BAF) complex. However, there are a number of patients with the clinical diagnosis of CSS or NCBRS in whom the causative mutation has not been ABT-737 inhibitor identified. In this study, we performed trio-based whole-exome sequencing (WES) in ten previously described but unsolved individuals with the tentative diagnosis of CSS or NCBRS and found causative mutations in nine out of ten individuals. Interestingly, our WES analysis disclosed overlapping differential diagnoses including Wiedemann-Steiner, Kabuki, and Adams-Oliver syndromes. In addition, most likely causative de novo mutations were identified in GRIN2A and SHANK3. Moreover, trio-based WES detected SMARCA2 and SMARCA4 deletions, which had not been annotated in a previous Haloplex target enrichment and next-generation sequencing of known CSS/NCBRS genes emphasizing the advantages of WES as a diagnostic tool.

The effect of the fraction of the phases, influence of the transformation temperatures, the holding time, and the stability of retained austenite on the mechanical properties of these two steels was critically analyzed with the help of X-ray diffraction, optical

https://www.selleckchem.com/products/Cyclopamine.html metallography, scanning electron microscopy, and atomic force microscopy. Finally, a remarkable combination of yield strength of the level of 1557 MPa with a total elongation of 15.5 pct was obtained.”
“Recently, a new clustering method called maximum margin clustering (MMC) was proposed. It extended the support vector machine (SVM) thoughts to unsupervised scenarios and had shown promising performances. Traditionally, it was formulated as a non-convex integer optimization problem which was difficult to solve. In order to alleviate Selleckchem 5-Fluoracil the computational burden, the efficient cutting-plane

MMC (CPMMC) [22] was proposed which solved the MMC problem in its primal. However, the CPMMC is restricted to linear kernel. In this paper, we extend the CPMMC algorithm to the nonlinear kernel scenarios, which is the proposed sparse kernel MMC (SKMMC). Specifically, we propose to solve an adaptive threshold version of CPMMC in its dual and alleviate its computational complexity by employing the cutting plane subspace pursuit (CPSP) algorithm [7]. Eventually, the SKMMC algorithm could work with nonlinear kernels at a linear computational complexity and a linear storage complexity. Our experimental results on several real-world data sets show that the SKMMC has higher accuracies than existing MMC

methods, and Z-IETD-FMK supplier takes less time and storage demands than existing kernel MMC methods.”
“Shibata S, Levine BD. Biological aortic age derived from the arterial pressure waveform. J Appl Physiol 110: 981-987, 2011. First published February 3, 2011; doi:10.1152/japplphysiol.01261.2010.-Indexes for arterial stiffness are, by their nature, influenced by the ambient blood pressure due to the curvilinear nature of arterial compliance. We developed a new concept of the “Modelflow aortic age,” which is, theoretically, not influenced by the ambient blood pressure and provides an easily understood context (biological vs. chronological age) for measures of arterial stiffness. The purpose of the present study was to validate this pressure-independent index for aortic stiffness in humans. Twelve sedentary elderly (65-77 yr), 11 Masters athletes (65-73 yr), and 12 sedentary young individuals (20-42 yr) were studied. Modelflow aortic ages were comparable with chronological ages in both sedentary groups, indicating that healthy sedentary individuals have age-appropriate aortas. In contrast, Masters athletes showed younger Modelflow aortic ages than their chronological ages.

..water hydrogen-bonded network with a few other water molecules. A few dynamical conformations or transition states involving direct (His159 ND1…Asp158 OD1) and water-mediated (His159 ND1…W-2…Asp158 https://www.selleckchem.com/products/cftrinh-172.html OD1) hydrogen-bonded complexes are envisaged from these studies.”
“Background: Gastroesophageal reflux disease (GERD) is associated with impaired epithelial barrier function that is regulated by cell-cell contacts. The aim of the study was to investigate the expression pattern of selected components involved in the

formation of tight junctions in relation to GERD.\n\nMethods: Eighty-four patients with GERD-related symptoms with endoscopic signs (erosive: n = 47) or without them (non-erosive: n = 37) as well as 26 patients lacking GERD-specific symptoms ERK signaling inhibitors as controls were included. Endoscopic and histological characterization of esophagitis was performed according to the Los Angeles and adapted Ismeil-Beigi criteria, respectively. Mucosal biopsies from distal esophagus were

taken for analysis by histopathology, immunohistochemistry and quantitative reverse-transcription polymerase chain reaction (RT-PCR) of five genes encoding tight junction components [Occludin, Claudin-1, -2, Zona occludens (ZO-1, -2)].\n\nResults: Histopathology confirmed GERD-specific alterations as dilated intercellular spaces in the esophageal mucosa of patients with GERD compared to controls (P < 0.05). Claudin-1 and -2 were 2- to 6-fold upregulation on transcript (P < 0.01) and in part on protein level (P < 0.015) in GERD, while subgroup analysis of revealed this upregulation for ERD only. In both erosive and non-erosive reflux disease, expression levels of Occludin and ZO-1,-2 were not significantly

affected. Notably, the induced expression of both claudins GSK2399872A clinical trial did not correlate with histopathological parameters (basal cell hyperplasia, dilated intercellular spaces) in patients with GERD.\n\nConclusions: Taken together, the missing correlation between the expression of tight junction-related components and histomorphological GERD-specific alterations does not support a major role of the five proteins studied in the pathogenesis of GERD.”
“Leigh syndrome is a mitochondrial disease with considerable clinical and genetic variation. We present a 16-year-old boy with Leigh-like syndrome and broad developmental retardation, parkinsonism and hypogonadism. Sequencing of the entire mitochondrial DNA from blood revealed the m.4296G>A mutation in the MT-TI gene. The mutation was heteroplasmic with a 95% proportion of the mutant genome, while the proportion was 58% in the blood of the patient’s clinically healthy mother. Our results suggest that m.4296G>A is pathogenic in humans, and that the phenotype related to this change includes Leigh-like syndrome in adolescence with parkinsonism and hypogonadism, in addition to the previously reported early infantile Leigh syndrome.

The detection result could be either qualitatively resolved with naked eye or quantitatively analyzed with the assistance of a digital

camera. In the qualitative mode, the limit of detection (LOD) was found to be 0.25 ng/mL. In the quantitative mode, the half-maximal inhibition concentration (IC50) was determined to be 0.45 ng/mL and the LOD can reach an ultralow level of 0.03 ng/mL, which is similar to 100 times lower than that of the conventional colloidal gold-based lateral flow immunoassay. Potential application of the established method was demonstrated by analyzing representative cow milk samples.”
“In a previous functional magnetic resonance imaging GDC 973 (fMRI) study, where we investigated the neural correlates of esthetic experience, we found the observing canonical sculptures, relative to sculptures whose proportions had been modified, produced the activation of a network that included the lateral occipital gyrus, precuneus, prefrontal areas, and, most interestingly, the right anterior insula. We interpreted this latter activation as the neural signature

underpinning Selleck Pitavastatin hedonic response during esthetic experience. With the aim of exploring whether this specific hedonic response is also present during the observation of non-art biological stimuli, in the present fMRI study we compared the activations associated with viewing masterpieces of classical sculpture with those produced by the observation of pictures of young athletes. The two stimulus-categories were matched on various factors, including body postures, proportion, and expressed dynamism. The stimuli were presented in two conditions: observation and esthetic judgment. The two stimulus-categories produced a rather similar global activation pattern. Direct comparisons between sculpture and real-body images revealed, however, relevant differences, among which the activation of right antero-dorsal insula during sculptures viewing only. Along with our previous data, this finding suggests that the

hedonic state associated with activation of right dorsal anterior insula underpins esthetic experience selleck compound for artworks.”
“The present study was designed to study the effect of different transplanting methods in comparison with the farmer’s conventional transplanting methods at farmer’s field. Experiments were laid out in randomized complete block design (RCBD) and replicated thrice having a net plot size of 30 m x 40 m. The studies were carried out consecutively for three years during kharif season 2003, 2004 and 2005. For this study fifteen(15) locations viz; Sheikhupura (Mallian Kalan), Nankana Sahib (Warburten), Lahore (Burki), Wazirabad (Manzoorbad), Wazirabad (Rakh Bharokae), Kamonke (Ramey Farm), Noshehra Virkan, Daska, Pindi Bhattian (Sokhakea), Hafizabad (Ahmadpur Chatha), Pasroor (Poorib Klair), Gujranwala (Batala farm), Norowal (Burj).